Estimating mesophyll conductance to CO2: methodology, potential errors, and recommendations
Identifieur interne : 000001 ( France/Analysis ); précédent : 000000; suivant : 000002Estimating mesophyll conductance to CO2: methodology, potential errors, and recommendations
Auteurs : Thijs L. Pons [Pays-Bas] ; Jaume Flexas [Espagne] ; Susanne Von Caemmerer [Australie] ; John R. Evans [Australie] ; Bernard Genty [France] ; Miquel Ribas-Carbo [Espagne] ; Enrico Brugnoli [Italie]Source :
- Journal of Experimental Botany [ 0022-0957 ] ; 2009-04-08.
Abstract
The three most commonly used methods for estimating mesophyll conductance (gm) are described. They are based on gas exchange measurements either (i) by themselves; (ii) in combination with chlorophyll fluorescence quenching analysis; or (iii) in combination with discrimination against 13CO2. To obtain reliable estimates of gm, the highest possible accuracy of gas exchange is required, particularly when using small leaf chambers. While there may be problems in achieving a high accuracy with leaf chambers that clamp onto a leaf with gaskets, guidelines are provided for making necessary corrections that increase reliability. All methods also rely on models for the calculation of gm and are sensitive to variation in the values of the model parameters. The sensitivity to these factors and to measurement error is analysed and ways to obtain the most reliable gm values are discussed. Small leaf areas can best be measured using one of the fluorescence methods. When larger leaf areas can be measured in larger chambers, the online isotopic methods are preferred. Using the large CO2 draw-down provided by big chambers, and the isotopic method, is particularly important when measuring leaves with high gm that have a small difference in [CO2] between the substomatal cavity and the site of carboxylation in the chloroplast (CiCc gradient). However, equipment for the fluorescence methods is more easily accessible. Carbon isotope discrimination can also be measured in recently synthesized carbohydrates, which has its advantages under field conditions when large number of samples must be processed. The curve-fitting method that uses gas exchange measurements only is not preferred and should only be used when no alternative is available. Since all methods have their weaknesses, the use of two methods for the estimation of gm, which are as independent as possible, is recommended.
Url:
DOI: 10.1093/jxb/erp081
Affiliations:
- Australie, Espagne, France, Italie, Pays-Bas
- Provence-Alpes-Côte d'Azur, Utrecht (province)
- Saint Paul lez Durance, Utrecht
- Université d'Utrecht
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000789
- to stream Istex, to step Curation: 000710
- to stream Istex, to step Checkpoint: 000087
- to stream Main, to step Merge: 000124
- to stream Main, to step Curation: 000124
- to stream Main, to step Exploration: 000110
- to stream France, to step Extraction: 000001
Links to Exploration step
ISTEX:8F57DD1D62918A7F61DDFCD169F19958C20E56D1Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Estimating mesophyll conductance to CO2: methodology, potential errors, and recommendations</title><author><name sortKey="Pons, Thijs L" sort="Pons, Thijs L" uniqKey="Pons T" first="Thijs L." last="Pons">Thijs L. Pons</name></author><author><name sortKey="Flexas, Jaume" sort="Flexas, Jaume" uniqKey="Flexas J" first="Jaume" last="Flexas">Jaume Flexas</name></author><author><name sortKey="Von Caemmerer, Susanne" sort="Von Caemmerer, Susanne" uniqKey="Von Caemmerer S" first="Susanne" last="Von Caemmerer">Susanne Von Caemmerer</name></author><author><name sortKey="Evans, John R" sort="Evans, John R" uniqKey="Evans J" first="John R." last="Evans">John R. Evans</name></author><author><name sortKey="Genty, Bernard" sort="Genty, Bernard" uniqKey="Genty B" first="Bernard" last="Genty">Bernard Genty</name></author><author><name sortKey="Ribas Carbo, Miquel" sort="Ribas Carbo, Miquel" uniqKey="Ribas Carbo M" first="Miquel" last="Ribas-Carbo">Miquel Ribas-Carbo</name></author><author><name sortKey="Brugnoli, Enrico" sort="Brugnoli, Enrico" uniqKey="Brugnoli E" first="Enrico" last="Brugnoli">Enrico Brugnoli</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:8F57DD1D62918A7F61DDFCD169F19958C20E56D1</idno><date when="2009" year="2009">2009</date><idno type="doi">10.1093/jxb/erp081</idno><idno type="url">https://api.istex.fr/document/8F57DD1D62918A7F61DDFCD169F19958C20E56D1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000789</idno><idno type="wicri:Area/Istex/Curation">000710</idno><idno type="wicri:Area/Istex/Checkpoint">000087</idno><idno type="wicri:doubleKey">0022-0957:2009:Pons T:estimating:mesophyll:conductance</idno><idno type="wicri:Area/Main/Merge">000124</idno><idno type="wicri:Area/Main/Curation">000124</idno><idno type="wicri:Area/Main/Exploration">000110</idno><idno type="wicri:Area/France/Extraction">000001</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Estimating mesophyll conductance to CO2: methodology, potential errors, and recommendations</title><author><name sortKey="Pons, Thijs L" sort="Pons, Thijs L" uniqKey="Pons T" first="Thijs L." last="Pons">Thijs L. Pons</name><affiliation wicri:level="4"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Plant Ecophysiology, Utrecht University, PO Box 80084, 3598 TB Utrecht</wicri:regionArea><orgName type="university">Université d'Utrecht</orgName><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName></affiliation></author><author><name sortKey="Flexas, Jaume" sort="Flexas, Jaume" uniqKey="Flexas J" first="Jaume" last="Flexas">Jaume Flexas</name><affiliation wicri:level="1"><country xml:lang="fr">Espagne</country><wicri:regionArea>Research Group on Plant Biology under Mediterranean Conditions, Department of Biology, Universitat de les Illes Balears, Carretera de Valldemossa Km 7.5, 07122 Palma de Mallorca, Illes Balears</wicri:regionArea><wicri:noRegion>Illes Balears</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Espagne</country></affiliation></author><author><name sortKey="Von Caemmerer, Susanne" sort="Von Caemmerer, Susanne" uniqKey="Von Caemmerer S" first="Susanne" last="Von Caemmerer">Susanne Von Caemmerer</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Research School of Biological Sciences, The Australian National University, Canberra, Australian Capital Territory 2601</wicri:regionArea><wicri:noRegion>Australian Capital Territory 2601</wicri:noRegion></affiliation></author><author><name sortKey="Evans, John R" sort="Evans, John R" uniqKey="Evans J" first="John R." last="Evans">John R. Evans</name><affiliation wicri:level="1"><country xml:lang="fr">Australie</country><wicri:regionArea>Research School of Biological Sciences, The Australian National University, Canberra, Australian Capital Territory 2601</wicri:regionArea><wicri:noRegion>Australian Capital Territory 2601</wicri:noRegion></affiliation></author><author><name sortKey="Genty, Bernard" sort="Genty, Bernard" uniqKey="Genty B" first="Bernard" last="Genty">Bernard Genty</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>CEA, CNRS, Universit Aix-Marseille, UMR 6191 Biologie Vgtale et Microbiologie Environnementale, Laboratoire d'Ecophysiologie Molculaire des Plantes, CEA Cadarache, 13108 Saint Paul lez Durance</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Saint Paul lez Durance</settlement></placeName></affiliation></author><author><name sortKey="Ribas Carbo, Miquel" sort="Ribas Carbo, Miquel" uniqKey="Ribas Carbo M" first="Miquel" last="Ribas-Carbo">Miquel Ribas-Carbo</name><affiliation wicri:level="1"><country xml:lang="fr">Espagne</country><wicri:regionArea>Research Group on Plant Biology under Mediterranean Conditions, Department of Biology, Universitat de les Illes Balears, Carretera de Valldemossa Km 7.5, 07122 Palma de Mallorca, Illes Balears</wicri:regionArea><wicri:noRegion>Illes Balears</wicri:noRegion></affiliation></author><author><name sortKey="Brugnoli, Enrico" sort="Brugnoli, Enrico" uniqKey="Brugnoli E" first="Enrico" last="Brugnoli">Enrico Brugnoli</name><affiliation wicri:level="1"><country xml:lang="fr">Italie</country><wicri:regionArea>CNR-Institute of Agro-Environmental Biology and Forestry, Via Marconi 2, I-05010 Porano (TR)</wicri:regionArea><wicri:noRegion>I-05010 Porano (TR)</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Botany</title><idno type="ISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2009-04-08">2009-04-08</date><biblScope unit="volume">60</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="2217">2217</biblScope><biblScope unit="page" to="2234">2234</biblScope></imprint><idno type="ISSN">0022-0957</idno></series><idno type="istex">8F57DD1D62918A7F61DDFCD169F19958C20E56D1</idno><idno type="DOI">10.1093/jxb/erp081</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-0957</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The three most commonly used methods for estimating mesophyll conductance (gm) are described. They are based on gas exchange measurements either (i) by themselves; (ii) in combination with chlorophyll fluorescence quenching analysis; or (iii) in combination with discrimination against 13CO2. To obtain reliable estimates of gm, the highest possible accuracy of gas exchange is required, particularly when using small leaf chambers. While there may be problems in achieving a high accuracy with leaf chambers that clamp onto a leaf with gaskets, guidelines are provided for making necessary corrections that increase reliability. All methods also rely on models for the calculation of gm and are sensitive to variation in the values of the model parameters. The sensitivity to these factors and to measurement error is analysed and ways to obtain the most reliable gm values are discussed. Small leaf areas can best be measured using one of the fluorescence methods. When larger leaf areas can be measured in larger chambers, the online isotopic methods are preferred. Using the large CO2 draw-down provided by big chambers, and the isotopic method, is particularly important when measuring leaves with high gm that have a small difference in [CO2] between the substomatal cavity and the site of carboxylation in the chloroplast (CiCc gradient). However, equipment for the fluorescence methods is more easily accessible. Carbon isotope discrimination can also be measured in recently synthesized carbohydrates, which has its advantages under field conditions when large number of samples must be processed. The curve-fitting method that uses gas exchange measurements only is not preferred and should only be used when no alternative is available. Since all methods have their weaknesses, the use of two methods for the estimation of gm, which are as independent as possible, is recommended.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Espagne</li><li>France</li><li>Italie</li><li>Pays-Bas</li></country><region><li>Provence-Alpes-Côte d'Azur</li><li>Utrecht (province)</li></region><settlement><li>Saint Paul lez Durance</li><li>Utrecht</li></settlement><orgName><li>Université d'Utrecht</li></orgName></list><tree><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="Pons, Thijs L" sort="Pons, Thijs L" uniqKey="Pons T" first="Thijs L." last="Pons">Thijs L. Pons</name></region></country><country name="Espagne"><noRegion><name sortKey="Flexas, Jaume" sort="Flexas, Jaume" uniqKey="Flexas J" first="Jaume" last="Flexas">Jaume Flexas</name></noRegion><name sortKey="Flexas, Jaume" sort="Flexas, Jaume" uniqKey="Flexas J" first="Jaume" last="Flexas">Jaume Flexas</name><name sortKey="Ribas Carbo, Miquel" sort="Ribas Carbo, Miquel" uniqKey="Ribas Carbo M" first="Miquel" last="Ribas-Carbo">Miquel Ribas-Carbo</name></country><country name="Australie"><noRegion><name sortKey="Von Caemmerer, Susanne" sort="Von Caemmerer, Susanne" uniqKey="Von Caemmerer S" first="Susanne" last="Von Caemmerer">Susanne Von Caemmerer</name></noRegion><name sortKey="Evans, John R" sort="Evans, John R" uniqKey="Evans J" first="John R." last="Evans">John R. Evans</name></country><country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Genty, Bernard" sort="Genty, Bernard" uniqKey="Genty B" first="Bernard" last="Genty">Bernard Genty</name></region></country><country name="Italie"><noRegion><name sortKey="Brugnoli, Enrico" sort="Brugnoli, Enrico" uniqKey="Brugnoli E" first="Enrico" last="Brugnoli">Enrico Brugnoli</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/MonteverdiV1/Data/France/Analysis
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000001 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/France/Analysis/biblio.hfd -nk 000001 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= MonteverdiV1
|flux= France
|étape= Analysis
|type= RBID
|clé= ISTEX:8F57DD1D62918A7F61DDFCD169F19958C20E56D1
|texte= Estimating mesophyll conductance to CO2: methodology, potential errors, and recommendations
}}
| This area was generated with Dilib version V0.6.21. |  |